| Autor: |
Buttery, Joseph E., Stuart, Sandra, Gee, David J. |
| Zdroj: |
Pathology; January 1991, Vol. 23 Issue: 1 p77-79, 3p |
| Abstrakt: |
A modification of the colorimetric method of Bergman and Loxley for the measurement of urinary hydroxyproline using external aqueous hydroxyproline standards instead of individual internal standards is described.1We show that this modification leads to an underestimation (average 32%) of hydroxyproline because suppression of colour development occurs in urine samples but not in aqueous standards. Use of an internal standard for each urine test corrects for this suppression. Recovery of hydroxyproline (250μmol/L) added to 12 patient urine samples averaged 99% and the overall imprecision for the assay was then <5%. Modifications to the original hydrolysis and colour development procedures allow linearity to 1500μmol/L Details of our procedure are given. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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