| Autor: |
Fornari, Gheniffer, Vicente, Vania Aparecida, Gomes, Renata Rodrigues, Muro, Marisol Dominguez, Pinheiro, Rosangela Lameira, Ferrari, Carolina, Herkert, Patricia Fernanda, Takimura, Marcos, Carvalho, Newton Sérgio de, Queiroz-Telles, Flavio |
| Zdroj: |
Brazilian Journal of Microbiology; April-June 2016, Vol. 47 Issue: 2 p373-380, 8p |
| Abstrakt: |
Vulvovaginal candidiasis affects women of reproductive age, which represents approximately 15–25% of vaginitis cases. The present study aimed to isolate and characterize yeast from the patients irrespective of the presentation of clinical symptoms. The isolates were subjected to in vitrosusceptibility profile and characterization by molecular markers, which intended to assess the distribution of species. A total of 40 isolates were obtained and identified through the CHROMagar, API20aux and by ITS and D1/D2 regions sequencing of DNAr gene. Candida albicansstrains were genotyped by the ABC system and the isolates were divided into two genotypic groups. The identity of the C. albicans, C. glabrata, C. guilliermondii, C. kefyrand Saccharomyces cerevisiaeisolates was confirmed by the multilocus analysis. The strains of Candida, isolated from patients with complications, were found to be resistant to nystatin but sensitive to fluconazole, amphotericin B and ketoconazole, as observed by in vitrosensitivity profile. The isolates from asymptomatic patients, i.e., the colonized group, showed a dose-dependent sensitivity to the anti-fungal agents, fluconazole and amphotericin B. However, the isolates of C. albicansthat belong to distinct genotypic groups showed the same in vitrosusceptibility profile. |
| Databáze: |
Supplemental Index |
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