A radioimmunoassay for chironomid hemoglobin

Autor: Trewitt, Patrick M., Bergtrom, Gerald, Hsiao, Ku-Chuan
Zdroj: Biochemical and Biophysical Research Communications; September 1982, Vol. 108 Issue: 2 p526-533, 8p
Abstrakt: A double antibody, competitive radioimmunoassay was developed for the quantitation of stage-specific hemoglobin from the insect Chironomus thummi. The radioimmunoassay will detect as little as 150 picograms of Hb 3, a hemoglobin synthesized and secreted into the hemolymph of larvae during the 4th, but not the 3rd instar. The assay also detects cross-reacting hemoglobins purified from 4th instar larvae and in freshly laid eggs. Application of this radioimmunoassay is discussed in light of the cross-reacting Hbs in the insect.
Databáze: Supplemental Index