Autor: |
Trewitt, Patrick M., Bergtrom, Gerald, Hsiao, Ku-Chuan |
Zdroj: |
Biochemical and Biophysical Research Communications; September 1982, Vol. 108 Issue: 2 p526-533, 8p |
Abstrakt: |
A double antibody, competitive radioimmunoassay was developed for the quantitation of stage-specific hemoglobin from the insect Chironomus thummi. The radioimmunoassay will detect as little as 150 picograms of Hb 3, a hemoglobin synthesized and secreted into the hemolymph of larvae during the 4th, but not the 3rd instar. The assay also detects cross-reacting hemoglobins purified from 4th instar larvae and in freshly laid eggs. Application of this radioimmunoassay is discussed in light of the cross-reacting Hbs in the insect. |
Databáze: |
Supplemental Index |
Externí odkaz: |
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