| Autor: |
Mosier, B., Molho, J., Santiago, J. |
| Zdroj: |
Experiments in Fluids; 20021001, Vol. 33 Issue: 4 p545-554, 10p |
| Abstrakt: |
Abstract. A photobleached-fluorescence imaging technique for visualizing microscale flow fields and obtaining molecular diffusion and advection information has been developed. The technique tracks fluorophores in the region of a photobleached line in a planar microdevice and yields quantitative diffusive and advective transport data. Visualizations of two- and weakly three-dimensional electroosmotically and pressure-driven fluid flow fields are demonstrated using the photobleaching of fluorescein and fluorescein-dextran conjugates. Photobleached-fluorescence imaging tracks undisturbed fluorophores, functions in polymer and glass microfluidic devices, can take advantage of fluorescent conjugates present in biochemical assays, and has a photobleached region that is flow independent. |
| Databáze: |
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