| Abstrakt: |
Cytokines are signaling proteins including chemokines, interleu-kins, interferons, lymphokines and tumor necrosis factor that induce immune-based inflammatory responses. Elevated levels of cytokines provoke a broad range of cellular and physiological response and inflammation. Opuntia dilleni(KER-GAW) HAW belongs to genus Opuntia (Fam. Cactaceae) and has shown remarkable effects as an anti inflammatory agent. In the present investigation we have evaluated novel compounds opuntiol and opuntioside-I derived from Opuntia dilleniwhich have shown significant decrease in various cytokines (interleukins) and che-mokines in a dose dependant manner. Cytokines, i.e., tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), interleukin 6 (IL-6), interleukin 10 (IL-10) and chemokines, i.e., keratinocyte chemottractant (KC), JE, and monocyte chemottractant factor-1 (MCP-1) were evaluated in the absence and presence of opuntiol and opuntioside-I administered orally 60 minutes prior to induction of peritonitis via carrageenan and zymosan, respectively, using ELIS A. At highest dose 20mg/kg opuntiol/opuntioside-I inhibited IL-1β, IL-6, TNF-α, JE, KC and MCP-1 by 50-90%, whereas IL-10 levels were unchanged in all doses. Additionally, marked decline in mRNA expressions of pro-inflammatory cytokines (TNF, IL-1β, IL-6, IL-10) and chemokines (KC and MCP-1) were observed after i.p. treatment with 20mg/kg of opuntiol/opuntioside-I during zymosan induced peritonitis using RT-PCR. IL-1β, IL-6 and TNF-α activating NF-KB signaling pathway induced by JAK/STAT and Ikβ pathways which proceed with upregulation of various inflammatory gene such as COX2 and iNOS, growth factors, cytokines etc. Hence, opuntiol/opuntioside-I inhibiting the levels and reducing the expression of aforementioned cytokines and chemokine can be a potential immunomodulatory agent against asthma, rheumatoid arthritis, atherosclerosis, chronic bronchitis, psoriasis, etc. |
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