| Autor: |
Selvam, Kandasamy, Sudhakar, Chinnappan, Govarthanan, Muthusamy, Thiyagarajan, Periasamy, Sengottaiyan, Arumugam, Senthilkumar, Balakrishnan, Selvankumar, Thangasamy |
| Zdroj: |
Journal of Radiation Research and Applied Sciences; January 2017, Vol. 10 Issue: 1 p6-12, 7p |
| Abstrakt: |
The present study reports an eco-friendly, rapid and easy method for synthesis of silver nanoparticles (AgNPs) using Tinospora cordifoliaas a reducing and capping agent. The different factor such as silver nitrate (AgNO3) concentration, fresh weight of T. cordifolialeaf, incubation time, and pH affecting silver reduction was investigated using Response surface methodology based Box–Behnken design (BBD). The optimum conditions were AgNO3(1.25 mM), incubation time (15 h), Temperature (45 °C) and pH (4.5). T. cordifolialeaf extract can reduces silver ions into AgNPs within 30 min after heating the reaction mixture (60 °C) as indicated by the developed reddish brown color. The UV-Vis spectrum of AgNPs revealed a characteristic surface plasmon resonance (SPR) peak at 430 nm. AgNPs were characterized X-ray diffraction (XRD) revealed their crystalline nature and their average size of nanoparticles was 30 nm as determined by using Scherrer's equation. Fourier transform infrared (FTIR) spectroscopy affirmed the role of T. cordifolialeaf extract as a reducing and capping agent of silver ions. Scanning electron microscope-energy dispersive X-ray spectroscopy (SEM-EDS) showed spherical shaped and confirming presence of elemental silver. The synthesized AgNPs was found higher antioxidant activity than plant extract by dot plot assay. In addition, antibacterial activity against Staphylococcussp. (NCBI-Accession: KC688883.1) and Klebsiellasp. (NCBI-Accession: KF649832.1), showed maximum zone of inhibition of 13 mm and 12.3 mm, respectively, at 10 μg/mL of AgNPs. From the results it is suggested that the synthesized AgNPs showed higher antioxidant and antibacterial activity than the plant extract, thus signification of the present study is the production of biomedical products. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
