| Autor: |
Turi, J.S., Higuchi, W.I., Shipman, C., Ho, N.F.H. |
| Zdroj: |
Journal of Pharmaceutical Sciences; October 1972, Vol. 61 Issue: 10 p1618-1624, 7p |
| Abstrakt: |
Following the guidelines provided by the physical models derived earlier, sensitive experimental techniques and procedures were developed. Baselines for solute transport studies were established from experiments on the uptake of cholesterol−11C by Burkitt lymphoma cells in culture suspension at pH 7.3 under iso‐osmotic conditions. The experimental data were evaluated by the model involving the rapid equilibration of the solute in the cell interior after permeation through the plasma membrane. The time‐dependent predictions of this model agreed well with the data. Effective permeability and partition coefficients were calculated and found to be inversely proportional to the serum level used. These results are in agreement with the mechanism by which only the unbound cholesterol in the external solution participates in the membrane transport process. |
| Databáze: |
Supplemental Index |
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