| Autor: |
Foss, Robert G., Sigel, Carl W. |
| Zdroj: |
Journal of Pharmaceutical Sciences; October 1982, Vol. 71 Issue: 10 p1176-1178, 3p |
| Abstrakt: |
Specific methods using high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) for the analysis of the potent antifolate, 2,4-diamino-6-(2,5-dimethoxybenzyl)-5- methylpyrido-[2,3-dJpyrimidine (I) in plasma were developed. The HPLC system employed paired-ion chromatography using a mobile phase of water-acetonitrile (65:35, v/v) in conjunction with a reversed-phase C-l column and detection by UV absorbance measurement. The TLC system utilized a scanning densitometer operating in the reflectance mode with detection of I by fluorescence measurement. The lower limits of detection for the HPLC and TLC methods were ~0.005 μg/ml. The coefficients of variation for the measurement of drug concentrations over the range of 0.04-1.0 μg/ml of plasma were 5 and 6%, respectively. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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