| Autor: |
Chong, Clifford W., Dittert, Lewis W., Kostenbauder, Harry B., Swintosky, Joseph V. |
| Zdroj: |
Journal of Pharmaceutical Sciences; December 1967, Vol. 56 Issue: 12 p1647-1652, 6p |
| Abstrakt: |
A study was undertaken to establish an experimental procedure whereby accurate rates of enzyme-catalyzed substrate hydrolysis could be measured with a pH stat when solutions of blood plasma are used as the enzyme source. pH stat procedures which neglect the effects of plasma deterioration and loss of CO2from plasma solutions yield apparent hydrolysis rates which are faster and slower, respectively, than the true rates. To overcome the problems of anomalous substrate hydrolysis rates and apparent nonstoichiometric titrations, the plasma solutions were prepared aseptically and the titration assembly from a Radiometer pH stat was modified to effect a virtually closed system. Hydrolysis rate data are presented for acetylcholine, benzoylcholine, and phenyl butyrate in 0.5 percent solutions of human blood plasma to demonstrate the utility of the proposed experimental procedure and the modified pH stat titration assembly. |
| Databáze: |
Supplemental Index |
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