| Autor: |
Viswanathan, V., Mahn, F.P., Venturella, V.S., Senkowski, B.Z. |
| Zdroj: |
Journal of Pharmaceutical Sciences; March 1970, Vol. 59 Issue: 3 p400-402, 3p |
| Abstrakt: |
A rapid and specific gas chromatographic method has been developed for the detection and determination of d-biotin. This technique has been found applicable to agricultural premixes and pharmaceutical injectable preparations. After suitable preliminary extraction, the biotin silyl ester is prepared using bis-(trimethylsilyl)acetamide (BSA) reagent. The silyl ester of d-biotin exhibited good peak symmetry and a linear response when utilizing a hydrogen flame ionization detector with a 2% OV-17 column operated at a temperature of 190°. The conditions for the assay of d-biotin in several preparations are described employing n-octa-cosane as the internal standard. The standard deviation of the developed procedure under the conditions studied was ±2.7%. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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