| Autor: |
Beamer, Robert L., Wynn, James E., Rollins, Janet R., Ledesma, Rafael E. |
| Zdroj: |
Journal of Pharmaceutical Sciences; August 1973, Vol. 62 Issue: 8 p1384-1386, 3p |
| Abstrakt: |
The effect of dimethyl sulfoxide upon the reactions catalyzed by lactate dehydrogenase was studied. Both the forward (lactate to pyruvate) and the reverse (pyruvate to lactate) reactions were investigated using dimethyl sulfoxide concentrations lower than those used by previous investigators. An aldol-type condensation of dimethyl sulfoxide with pyruvate is proposed as an explanation for the observation that the forward reaction is accelerated and the reverse reaction is slowed by the presence of dimethyl sulfoxide in the reaction medium. A product of the aldol-type condensation was identified in the enzyme reaction medium. The aldol condensation product was synthesized and determined to be 2-hydroxy-2-methyl-3-methylsulfinylpropanoic acid. |
| Databáze: |
Supplemental Index |
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