| Autor: |
Hadley, William M., Bousquet, William F., Miya, Tom S. |
| Zdroj: |
Journal of Pharmaceutical Sciences; January 1974, Vol. 63 Issue: 1 p57-59, 3p |
| Abstrakt: |
A previously reported enzymatic-fluorometric assay for reduced glutathione and oxidized glutathione was modified through the use of heat precipitation of sample protein and hydrogen peroxide oxidation of reduced glutathione to oxidized glutathione. These modifications were made to eliminate two critical pH adjustments present in the original assay and the possibility of interference with the assay of oxidized glutathione by the perchloric acid protein precipitant and the cupric-ion oxidant used in the original assay. The reduced glutathione and oxidized glutathione content of Harding-Passey melanoma was determined using the modified assay. The reduced glutathione content was similar to literature values. The precision of the assay was not adversely affected by these modifications. |
| Databáze: |
Supplemental Index |
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