| Abstrakt: |
At high arachidonic acid concentrations (164 μM) and without exogenous cofactors, ram seminal vesicle microsomes produced prostaglandin E2and two less polar products, identified as prostaglandin D2and 15-ketoprostaglandin E2. The ratio of the biosynthetic products formed depended on the exogenous cofactor and on the arachidonic acid concentration. At high arachidonic acid concentrations (>150 μM), tryptophan, phenol, and glutathione stimulated prostaglandin E2formation, but each affected the formation of the other prostaglandins differently. Ascorbic acid markedly stimulated hydroxy fatty acid formation. GLC-mass spectral analysis of the hydroxy fatty acid fraction indicated the presence of 11-hydroxy-5,8,12,14-eicosatetraenoic acid, 15-hydroxy-5,8,11,13-eicosatetraenoic acid, and 12-hydroxy-5,8,10-heptadecatrienoic acid. At low arachidonic acid concentrations (30 μM). glutathione still stimulated prostaglandin E2biosynthesis, but the other cofactors stimulated 6-ketoprostaglandin F1α”︁ and hydroxy fatty acid formation. |
Plný text