| Autor: |
Randolph, W.C., Osborne, V.L., Intoccia, A.P. |
| Zdroj: |
Journal of Pharmaceutical Sciences; November 1979, Vol. 68 Issue: 11 p1451-1453, 3p |
| Abstrakt: |
A method is described for the extraction of ticrynafen, a new hypotensive agent, and its reduced metabolite from serum and urine. Drug-related material is extracted from biological fluids with ether under strongly acidic conditions and then back-extracted into an alkaline aqueous phase, which is subjected to high-pressure liquid chromatographic analysis. Separations are performed on a reversed-phase column with a mobile phase consisting of phosphate buffer-acetonitrile. This accurate and reproducible method measures serum concentrations of ticrynafen and its reduced metabolite as low as 1.0 and 0.4 μg/ml, respectively. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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