| Abstrakt: |
The analysis of tetracycline epimers in tetracycline preparations by high-performance liquid chromatography is described. The method uses a microparticulate phenyl column (3.9mm i.d. × 30cm) with a step gradient of 12–22% acetonitrile in 0.2 Mphosphate buffer at pH 2.2. The analysis takes 22min. The relative standard deviations of the method (2σ, n = 6) for the analysis of chlortetracycline, 4-epitetracycline, 4-epianhydrotetracycline, and anhydrotetracycline in tetracycline were ±3.68, ±4.47, ±7.60, and ±2.77%, respectively. |
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