| Autor: |
Pantarotto, C., Salmona, M., Fanelli, R., Bianchi, M., Szczawinska, K. |
| Zdroj: |
Journal of Pharmaceutical Sciences; August 1981, Vol. 70 Issue: 8 p871-874, 4p |
| Abstrakt: |
A specific and sensitive method is described for the determination of saccharin in biological fluids. The compound is extracted as its methyl derivative following a salt-solvent pair procedure and assayed by GLC with either flame-ionization or mass fragmentographic detection using ethylated or trideuteromethylated saccharin, respectively, as the internal standard for quantitation. Detector response was linear over concentrations of 50 ng/ml-10 μg/ml with multiple-ion detection mass fragmentography and from 2 μg/ml up to milligram levels with flame-ionization detection. Interference from endogenous substrates was never observed. Plasma kinetics and urinary elimination of saccharin in healthy human volunteers given the sweetener orally, acutely (SO mg/60 kg of body weight) or for 5 days (130 mg/60 kg of body weight/day divided over the three main meals), also are reported. |
| Databáze: |
Supplemental Index |
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