Does the Capsule Interfere with Performance of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Cryptococcus neoformansand Cryptococcus gattii?

Autor: Thomaz, Danilo Y., Grenfell, Rafaella C., Vidal, Monica S. M., Giudice, Mauro C., Del Negro, Gilda M. B., Juliano, Luiz, Benard, Gil, de Almeida Júnior, João N.
Zdroj: Journal of Clinical Microbiology; November 2015, Vol. 54 Issue: 2 p474-477, 4p
Abstrakt: ABSTRACTWe described the impact of the capsule size for Cryptococcus neoformansand Cryptococcus gattiiidentification at the species level by Bruker matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS). After experimental capsule size modulation, we observed that reducing the capsule size resulted in improved identification by Bruker MALDI-TOF MS across all of the reference strains analyzed.
Databáze: Supplemental Index