| Autor: |
Manca, Laura, Ferranti, Pasquale, Barone, Francesca, Nitti, Gianpaolo, Malorni Piero Pucci, Antonio, Naitana, Salvatore, Masala, Bruno |
| Zdroj: |
International Journal of Biochemistry; January 1993, Vol. 25 Issue: 12 p1935-1938, 4p |
| Abstrakt: |
1.1. The amino acid substitution which characterizes the haemoglobin I variant from sheep has been ascertained using a combination of Fast Atom Bombardment mass spectrometry and protein sequencing.2.2. A Ser for Gly substitution at position 13 (10 of the A helix) was found in a polypeptide with the overall sequence of the βBglobin.3.3. On the basis of the nucleotide sequence of the βB-globin gene, a C to T transition occurring on a CpG doublet is considered to be responsible for the amino acid substitution.4.4. This represents the first observation of a variant sheep Hb due to a mutation which is rather common in the human genome.5.5. Amongst ruminants, serine is normally present at position 13 of goat and sheep ε11and γ chains and of bovine γ chain which had an independent and more ancient evolutionary origin than the β chains. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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