| Abstrakt: |
Verticillium biguttatum, a mycoparasite of the ubiquitous soil-borne plant pathogen Rhizoctonia solani, excreted chitinase and β-1,3-glucanase into liquid medium when grown on laminarin and chitin, respectively. Neither chitinase nor β-1,3-glucanase was produced by the mycoparasite when grown on cell walls of two isolates of R. solanirepresenting anastomosis groups (AG)-3 and AG-8. Extracellular protease was induced by growth on cell walls of the pathogen, whereas β-1,3-glucanase and chitinase were produced bound to the cell wall of V. biguttatum. This is the first report of chitinase, β-1,3-glucanase and protease production by V. biguttatum. These enzymes may play a previously unforeseen role in dissolving and penetrating the cell walls of R. solani. |
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