| Autor: |
Ireland, Ian D., Lewis, Darren F., Li, Xing-Fang, Renborg, Annika, Kwong, Sarah, Chen, Min, Dovichi, Norman J. |
| Zdroj: |
Journal of Protein Chemistry; July 1997, Vol. 16 Issue: 5 p491-493, 3p |
| Abstrakt: |
Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is a promising new method for the analysis of protein sequencing products. It gives 10 zmol (1 zmol = 10−21mol) limits of detection (3σ) for fluorescein thiohydantoin (FTH) amino acids. We have developed a separation for the (FTH)-amino acid products generated from 18 of the 20 coded amino acids. The extremely low volume requirement associated with CE-LIF makes it incompatible with commercial sequencers. For this reason, we have also been developing a miniaturized sequencer that can be more easily coupled to our detection system. Both the CE-LIF system and the miniaturized sequencer are described. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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