| Autor: |
Lyukmanova, E., Shulga, A., Arsenieva, D., Pluzhnikov, K., Dolgikh, D., Arseniev, A., Kirpichnikov, M. |
| Zdroj: |
Russian Journal of Bioorganic Chemistry; January 2004, Vol. 30 Issue: 1 p25-34, 10p |
| Abstrakt: |
Neurotoxin II from the venom of cobra Naja oxianais a short Type α-neurotoxin, which competitively inhibits nicotinic acetylcholine receptor. The toxin gene was expressed as a construct fused with the thioredoxin gene and the linker encoding the enteropeptidase recognition site and a Met residue between the genes. The fusion protein was mainly cleaved by cyanogen bromide, since enteropeptidase was less effective. The yield of neurotoxin II was 6 mg/l of the bacterial culture. The resulting recombinant protein was identified with native neurotoxin II by its N-terminal analysis, mass spectrometry, and NMR spectroscopy. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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