Completion of the Nucleotide Sequence of the Enterococcus faecalisConjugative Virulence Plasmid pAD1 and Identification of a Second Transfer Origin

Autor: Francia, M.Victoria, Haas, Wolfgang, Wirth, Reinhard, Samberger, Elke, Muscholl-Silberhorn, Albrecht, Gilmore, Michael S., Ike, Yasuyoshi, Weaver, Keith E., An, Florence Y., Clewell, Don B.
Zdroj: Plasmid; September 2001, Vol. 46 Issue: 2 p117-127, 11p
Abstrakt: pAD1 is a 59.3-kb plasmid in Enterococcus faecalisthat has been the subject of intense investigation with regard to its pheromone-inducible conjugation behavior as well as its contribution to virulence. Approximately two-thirds of the pAD1 nucleotide sequence has been previously reported. Here we report on an analysis of the final ∼22 kb, a significant portion of which is believed to encode structural genes associated with conjugation. The conjugation-related region was also found to contain a new (second) origin of conjugative transfer (oriT). A list of open reading frames covering the entire plasmid is presented.
Databáze: Supplemental Index
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