The Agrobacteriumoncogene AB-6bcauses a graft-transmissible enation syndrome in tobacco

Autor: Helfer, Anne, Clément, Bernadette, Michler, Pierre, Otten, Léon
Zdroj: Plant Molecular Biology; May 2003, Vol. 52 Issue: 2 p483-493, 11p
Abstrakt: Agrobacterium 6boncogenes induce tumours and modify plant growth in various ways. Here we show that the AB-6bgene from strain AB4 placed under 2x35S promoter control (2x35S-AB-6b) induces a complex enation syndrome in transgenic Nicotiana tabacumplants, that also occurs in a few rare cases of genetic enations. In Arabidopsis thaliana, 2x35S-AB-6binduced radially symmetrical tubes on the abaxial side of the leaves, which must therefore be considered as the Arabidopsisequivalents of enations on other plant species. Tobacco and Arabidopsis2x35S-AB-6bleaves contained small, supernumerary densely packed cells between the spongy mesophyll and the abaxial epidermis, close to vascular strands arising at an early stage of leaf development. On tobacco, the 2x35S-AB-6benation syndrome could be transmitted across graft junctions to growing tissues of untransformed plants, both acropetally and basipetally. We propose that the AB-6bgene encodes the synthesis of one or more enation factor(s) that are transported by the phloem and modify the growth of developing tissues.
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