| Autor: |
Semyanov, Konstantin A., Tarasov, Peter A., Zharinov, Alexey E., Chernyshev, Andrei V., Hoekstra, Alfons G., Maltsev, Valeri P. |
| Zdroj: |
Applied Optics; September 2004, Vol. 43 Issue: 26 p5110-5115, 6p |
| Abstrakt: |
A Fourier transform was applied to size an individual spherical particle from an angular light-scattering pattern. The position of the peak in the amplitude spectrum has a strong correlation with the particle size. A linear equation retrieved from regression analysis of theoretically simulated patterns provides a relation between the particle size and the location of the amplitude spectrum’s peak. The equation can be successfully applied to characterize particles of size parameters that range from 8 to 180 (corresponding to particle sizes that range from 1.2 to 27.2 μm at a wavelength of 0.633 μm). The precision of particle sizing depends on the refractive index and reaches a value of 60 nm within refractive-index region from 1.35 to 1.70. We have analyzed four samples of polystyrene microspheres with mean diameters of 1.9, 2.6, 3.0, and 4.2 μm and a sample of isovolumetrically sphered erythrocytes with a scanning flow cytometer to compare the accuracy of our new method with that of others. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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