Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli

Autor: Yavuz, Elif, Gunes, Hatice, Harsa, Sebnem, Bulut, Cisem, Fazil yenidunya, A.
Zdroj: World Journal of Microbiology and Biotechnology; November 2004, Vol. 20 Issue: 8 p871-874, 4p
Abstrakt: Although thermophilic members of the genus Bacillusare known to tolerate extreme environmental conditions, they appeared to be readily damaged upon mechanical manipulations. This fact was evidenced in genotyping of some strains of thermophilic Bacillusby pulsed field gel electrophoresis (PFGE). Consequently, a new procedure for the preparation of agarose plugs was developed. The procedure produced interpretable genomic DNA restriction profiles.
Databáze: Supplemental Index