| Autor: |
Miranda, D.M., Romano-Silva, M.A., Kalapothakis, E., Diniz, C.R., Cordeiro, M.N., Moraes-Santos, T., Marco, L. De, Prado, M.A.M., Gomez, M.V. |
| Zdroj: |
Brain Research Bulletin; 2001, Vol. 54 Issue: 5 p533-536, 4p |
| Abstrakt: |
In this paper we describe the effects of the β scorpion toxin Tityus γ (TiTX γ) and spider neurotoxins Tx3-3 and Tx3-4 in the Ca uptake in synaptosomes. The TiTX γ-stimulatory effect on Ca uptake in synaptosomes was inhibited ω-Conotoxin MVIIC (ω-CgTX MVIIC) (0.1 μM) and ω-Agatoxin IVA (0.1 μM) by 70% and 41%, respectively. ω-CgTX MVIIC (1.0 μM) almost completely blocked the TiTX γ-induced Ca uptake in synaptosomes. Verapamil (1.0 μM) and ω-Conotoxin GVIA (0.1 μM) had no effect in the scorpion toxin-induced Ca influx. The spider neurotoxins Tx3-3 and Tx3-4 inhibited the TiTX γ-induced calcium uptake with an IC 50 of 10.0 and 30.0 nM, respectively. It is suggested that spider neurotoxins Tx3-3 and Tx3-4 blocking effect in the TiTX γ-induced calcium uptake involves P/Q-type calcium channels. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect