Autor: |
Van Tol, Maarten J.D., Veenhoff, Egbert, Seijen, Hendrik G. |
Zdroj: |
Journal of Immunological Methods; May 1978, Vol. 21 Issue: 1-2 p125-131, 7p |
Abstrakt: |
Rabbit sera contain little IgM. This is a major obstacle for its isolation. However, levels of IgM up to 10 mg/ml are found in rabbits infected with living trypanosomes. A simple procedure, involving gel filtration, standing in the cold, and agarose block electrophoresis is described which allows the isolation of IgM from these sera in 60% yield, with a purity of better than 90%. |
Databáze: |
Supplemental Index |
Externí odkaz: |
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