Rabbit IgM: its isolation in high yield by a convenient procedure using serum from trypanosome-infected animals

Autor: Van Tol, Maarten J.D., Veenhoff, Egbert, Seijen, Hendrik G.
Zdroj: Journal of Immunological Methods; May 1978, Vol. 21 Issue: 1-2 p125-131, 7p
Abstrakt: Rabbit sera contain little IgM. This is a major obstacle for its isolation. However, levels of IgM up to 10 mg/ml are found in rabbits infected with living trypanosomes. A simple procedure, involving gel filtration, standing in the cold, and agarose block electrophoresis is described which allows the isolation of IgM from these sera in 60% yield, with a purity of better than 90%.
Databáze: Supplemental Index