| Autor: |
Kim, Yung D., Tomita, Joseph T., Schenck, Jay R. |
| Zdroj: |
Journal of Immunological Methods; March 1978, Vol. 19 Issue: 4 p309-316, 8p |
| Abstrakt: |
A simple and rapid solid-phase radioimmunoassay for the measurement of carcinoembryonic antigen (CEA) levels in sera or plasma is described. The procedure involves perchloric acid (PCA) extraction of the samples, followed by a rapid neutralization of excess acid with alkaline buffer solution. The PCA extracts are assayed using antibody-coated plastic tubes and radio-labeled anti-CEA antibody as a marker. The assay may be completed in one day. The solid-phase direct-binding assay demonstrated a sensitivity of 0.5 ng CEA/ml plasma and a marked tolerance to variations in pH and ionic strength of the system. A fair correlation between the CEA levels determined by the solid-phase radioimmunoassay and zirconium phosphate gel method was observed. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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