| Autor: |
Grenda, Tomasz, Kukier, Elżbieta, Goldsztejn, Magdalena, Kwiatek, Krzysztof, Kozieł, Nina |
| Zdroj: |
Bulletin of the Veterinary Institute in Pulawy; September 2015, Vol. 59 Issue: 3 p377-382, 6p |
| Abstrakt: |
As the test material mink feed with natural microflora was used. The analyses were conducted using Wrzosek and TPGY broth media, and Willis–Hobbs and Zeissler differential agar media. Wrzosek, Willis–Hobbs, and Zeissler media are described in Polish Standards approved by the National Standards Body in Poland and routinely used in detection of anaerobic bacteria in Poland. Detection and identification of C. botulinumwas performed with a previously validated real-time PCR method based on ntnhgene detection, which is common in all C. botulinumtoxotypes. The use of Wrzosek broth and Zeissler agar in routine analyses for detection and identification of C. botulinumwas ineffective and limited. The obtained results showed the highest culturing process effectiveness in TPGY broth with 72 h incubation at 30°C and isolation on Willis–Hobbs agar. The real-time PCR method based on ntnhgene detection used in this study could be utilised as a supplementary tool to the mouse lethality assay. |
| Databáze: |
Supplemental Index |
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