Protease-catalyzed incorporation of non-protein amino acids into peptides via the kinetically controlled approach

Autor: Miyazawa, Toshifumi, Hamahara, Kazumi, Matsuoka, Mikiko, Shindo, Yoshimi, Yamada, Takashi
Zdroj: Biotechnology Letters; April 1998, Vol. 20 Issue: 4 p389-392, 4p
Abstrakt: Through the kinetically controlled approach, employing the methyl ester as an acyl donor, non-protein aliphatic amino acids were incorporated into peptides using papain in an aqueous buffer, while the incorporation of non-protein aromatic amino acids was achieved by exploiting α-chymotrypsin in an aqueous buffer or acetonitrile with low water content. © Rapid Science Ltd. 1998
Databáze: Supplemental Index
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