| Autor: |
Dormeyer, Matthias, Schöneck, Ralf, Dittmar, Gunnar A.G., Krauth-Siegel, R. Luise |
| Zdroj: |
FEBS Letters; January 1997, Vol. 414 Issue: 2 p449-453, 5p |
| Abstrakt: |
Ribonucleotide reductase (RR) is an attractive drug target molecule. The gene of the R2 protein of Trypanosoma bruceiRR ( nrd B) has been cloned. It encodes a protein of 337 residues which shows about 60% identity with other eukaryotic R2 proteins. All residues which bind the iron center, the tyrosyl radical or are supposed to participate in the radical transfer are conserved in the trypanosomal protein sequence. Overexpression of the gene in E. coliresulted in 2–5 mg pure R2 protein from 100 ml bacterial cell culture. Northern blot analysis revealed a transcript of 1.85 kb in bloodstream and procyclic forms of the parasite. |
| Databáze: |
Supplemental Index |
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