| Autor: |
Chen, Yu-An, Obliosca, Judy M., Liu, Yen-Liang, Liu, Cong, Gwozdz, Mary L., Yeh, Hsin-Chih |
| Zdroj: |
Journal of the American Chemical Society; 20240101, Issue: Preprints |
| Abstrakt: |
While N6-methyladenine (m6A) is a common modification in prokaryotic and lower eukaryotic genomes and has many biological functions, there is no simple and cost-effective way to identify a single N6-methyladenine in a nucleic acid target. Here we introduce a robust, simple, enzyme-free and hybridization-based method using a new silver cluster probe, termed methyladenine-specific NanoCluster Beacon (maNCB), which can detect single m6A in DNA targets based on the fluorescence emission spectra of silver clusters. Not only can maNCB identify m6A at the single-base level but it also can quantify the extent of adenine methylation in heterogeneous samples. Our method is superior to high-resolution melting analysis as we can pinpoint the location of m6A in the target. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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