Autor: |
Behroozian, Adam A., Chludzinski, Jeffrey P., Lo, Eugene S., Ewing, Sarah A., Waslawski, Sheila, Newton, Duane W., Young, Vincent B., Aronoff, David M., Walk, Seth T. |
Zdroj: |
Infection Control & Hospital Epidemiology; September 2013, Vol. 34 Issue: 9 p961-966, 6p |
Abstrakt: |
Objective.To investigate the simultaneous occurrence of more than 1 Clostridium difficileribotype in patients' stool samples at the time of diagnostic testing.Methods.Stool samples submitted for diagnostic testing for the presence of toxigenic C. difficilewere obtained for 102 unique patients. A total of 95 single colonies of C. difficileper stool sample were isolated on selective media, subcultured alongside negative (uninoculated) controls, and polymerase chain reaction (PCR) ribotyped using capillary gel electrophoresis.Results.Capillary-based PCR ribotyping was successful for 9,335 C. difficileisolates, yielding a median of 93 characterized isolates per stool sample (range, 69-95). More than 1 C. difficileribotype was present in 16 of 102 (16%) C. difficileinfection (CDI) cases; 2 of the 16 mixtures were composed of at least 3 ribotypes, while the remaining 14 were composed of at least 2.Conclusions.Deep sampling of patient stool samples coupled with capillary-based PCR ribotyping identified a high rate of mixed CDI cases compared with previous estimates. Studies seeking to quantify the clinical significance of particular C. difficileribotypes should account for mixed cases of disease. |
Databáze: |
Supplemental Index |
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