| Autor: |
Monastryska, Iryna, Kiel, Jan A.K.W., Krikken, Arjen M., Komduur, Janet A., Veenhuis, Marten, Klei, Ida J. van der |
| Zdroj: |
Autophagy; July 2005, Vol. 1 Issue: 2 p92-100, 9p |
| Abstrakt: |
We have isolated the Hansenula polymorpha ATG11and ATG25genes, which are both required for glucose-induced selective peroxisome degradation (macropexophagy). ATG11was identified before in other yeast species and shown to be involved in the Cvt pathway in Saccharomyces cerevisiaeand glucose-induced micropexophagy in Pichia pastoris. Our data indicate that HpATG11is required for macropexophagy. ATG25represents a novel gene that encodes a 45 kDa coiled-coil protein. We show that this protein co-localizes with Atg11 on a small structure, which most likely represents the pre-autophagosomal structure (PAS). Cells of a constructed ATG25deletion strain (atg25) displayed relatively slow, continuous degradation of peroxisomes by microautophagy during growth on methanol in the presence of excess nitrogen that also continued after induction of selective peroxisome degradation. This suggests that the processes of selective and non-selective autophagy are dysregulated in atg25cells. |
| Databáze: |
Supplemental Index |
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