Application of atomic force microscopy for investigation of Na+,K+-ATPase signal-transducing function

Autor: Khalisov, MM, Ankudinov, AV, Penniyaynen, VA, Dobrota, D, Krylov, Boris V.
Zdroj: Acta Physiologica Hungarica; June 2015, Vol. 102 Issue: 2 p125-130, 6p
Abstrakt: The Young’s modulus of 10–12-day-old chick embryos’ sensory neurons cultivated in dissociated cell culture was measured using a PeakForce Quantitative Nanomechanical Mapping atomic force microscopy. The native cells were tested in control experiments and after application of ouabain. At low “endogenous” concentration of 10−10M, ouabain tended to increase the rigidity of sensory neurons. We hypothesize that this trend resulted from activation of Na+,K+-ATPase signal-transducing function.
Databáze: Supplemental Index