Comparison of hexamethyldisilazane and critical point drying treatments for SEM analysis of anaerobic biofilms and granular sludge

Autor: Araujo, Juliana C., Téran, Francisco C., Oliveira, Roberto A., Nour, Edson A. A., Montenegro, Martha A. P., Campos, José R., Vazoller, Rosana F.
Zdroj: Journal of Electron Microscopy; September 2003, Vol. 52 Issue: 4 p429-429, 1p
Abstrakt: We present a fast procedure for scanning electron microscopy (SEM) analysis in which hexamethyldisilazane (HMDS) solvent, instead of the critical point drying, is used to remove liquids from a microbiological specimen. The results indicate that the HMDS solvent is suitable for drying samples of anaerobic cells for examination by SEM and does not cause cell structure disruption.
Databáze: Supplemental Index