| Autor: |
Ridley, Robert G., White, John H., McAleese, Sybil M., Goman, Michael, Alano, Pietro, de Vries, Erik, Kilbey, Brian J. |
| Zdroj: |
Nucleic Acids Research; December 1991, Vol. 19 Issue: 24 p6731-6731, 1p |
| Abstrakt: |
Genes encoding proteins homologous to the catalytic subunifs of DNA polymerase α and δ have been cloned from the human malaria parasite Plasmodlum falclparum. These are among the first cellular replicative DNA polymerase genes to be cloned and their sequences allow us to make new statements about the relative degrees of conservation of these two enzymes. The most important finding was that P. falclparum Pol δ showed considerable homology to the only other Pol δ enzyme for which published sequence Is available, that of S. cerevlslae, displaying an overall amino acid identity of 45% and identity over a highly conserved central region of 59%. In contrast, the level of identity shown over the equivalent central region of Pol α between the P. falclparum and S. cerevlslae sequences Is only 32%The sequence data also allowed us to examine the degree of conservation in putative exonuclease domains of Pol δ. The Pol δ gene of P. falclparum maps to chromosome 10 and evidence is presented for the presence of different sized Pol δ mRNA's in the asexual and sexual erythrocytic stages of parasite development. |
| Databáze: |
Supplemental Index |
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