| Autor: |
Garson, Jeremy A., van den Berghe, Janette A., Kemshead, John T. |
| Zdroj: |
Nucleic Acids Research; June 1987, Vol. 15 Issue: 12 p4761-4761, 1p |
| Abstrakt: |
A novel in situ hybridization technique is described. This non-radioactive technique combines, for the first time, the high spacial resolution and rapid signal development of the non-isotopic approach with the previously unrivalled sensitivity of autoradiography. The procedure, which employs biotin labelled DBA probes and a streptavidin-alkaline phosphatase based detection system, is compatible with pre G-banding and can be performed on archival material. Unique sequences as small as 1 Kb are detectable. Using this technique, we have mapped the N-myc oncogene and the gene for β-Nerve Growth Factor to 2p24 and 1p13 respectively. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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