| Autor: |
DAVEY, M. R., KOTHARI, S. L., ZHANG, H., RECH, E. L., COCKING, E. C., LYNCH, P. T. |
| Zdroj: |
Journal of Experimental Botany; September 1991, Vol. 42 Issue: 9 p1159-1159, 1p |
| Abstrakt: |
Thirty eight green and 2 albino plants were regenerated from 400 kanamycin-resistant colonies derived from protoplasts isolated from cell suspensions of Oryza sativa variety Taipei 309 and electroporated with pCaMVNEO carrying the neomycin phosphotransferase II (nptII) gene. Twenty of the green transgenic Ro plants were transferred to the glasshouse, where 3 flowered after 7 months. Of 15 plants analysed by DNA hybridization, all carried the nptll gene, but only 2 of 11 plants assayed for NPTII activity expressed the nptll gene. One transgenic Ro plant produced 59 seeds following self-pollination. The seeds, when germinated on medium containing kanamycin sulphate, gave 16 green transgenic R, plants. Five transgenic R1 plants flowered and set seed, 7 flowered but failed to produce seeds, while 4 did not produce panicles. Transgenic Ro and R1 plants were shorter, required longer to flower, and had reduced pollen viability compared to non-transformed R1 protoplast-derived plants. The nptII gene was present in all 16 transgenic R1 plants, but NPTII activity was detected in only 8 of these plants. |
| Databáze: |
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