| Abstrakt: |
The effect of the acantholytic agent, cantharidin, on several biosynthetic systems was examined with epidermal tissue slices. Cantharidin is an effective inhibitor of protein synthesis, it stimulates RNA synthesis, and it leads to extensive phosphorylation of proteins. The inhibition of protein synthesis and the stimulation of RNA synthesis are probably reactions secondary to those which lead to acantholysis. The phosphorylation of proteins, on the other hand, appears to have a causal role in acantholysis. This reaction requires ATP, and its increased rate was induced only by cantharidin during acantholysis. Acantholysis leads to a threefold increase in soluble protein, and most of the phosphoprotein is in this fraction. |
