Effect of Intrinsic Factor on Vit. B12Uptake by Rat Intestine in vitro.∗

Autor: Strauss, Elliott W., Wilson, T. Hastings
Zdroj: Experimental Biology and Medicine; October 1958, Vol. 99 Issue: 1 p224-226, 3p
Abstrakt: Sacs of everted small intestine of the rat incubated in bicarbonate-saline containing glucose and B12showed only a very small B12uptake in the absence of a source of intrinsic factor. In the presence of neutralized rat gastric juice the stimulation of B12uptake was 10- to 25-fold. No B12appeared on the serosal side of the preparation. Stimulation of B12uptake was most marked in segments of intestine taken from the midportion of the small intestine. Evidence is presented that neither B12nor intrinsic factor when added alone are adsorbed to the intestinal wall but must be present simultaneously to give B12uptake.
Databáze: Supplemental Index