| Autor: |
Kirchner, F. R., Bybee, J. W., Osborne, J. W. |
| Zdroj: |
Experimental Biology and Medicine; April 1978, Vol. 157 Issue: 4 p572-575, 4p |
| Abstrakt: |
Any investigator assumes a priorithat data derived from studies utilizing different ages of animals cannot be rigorously compared. Several papers dealing with the compensatory response to intestinal resection have utilized animals of quite different age and weight and rarely has the stock been the same (see Table I). This study was designed to test the premise that the number of crypts per small intestine varies with animal weight and that indigenous crypt number will influence some features of the compensatory response of the crypt and villus cell population normally seen after intestinal resection (1-3).A reduction in the total villus and crypt cell population by surgical removal of a portion of the intestine is soon followed by compensatory responses, the most prominent being alteration in values for several parameters of mucosal cell kinetics (2, 3).The compensatory response to partial resection was assessed by the determination of (a) proliferative activity per crypt, (b) proliferative activity per milligram of tissue, (c) the number of crypts per milligram of tissue, and (d) the amount of tritiated thymidine (3HTdR) incorporated per epithelial cell nucleus. The values obtained from the above measurements were compared with values obtained from control animals and those with varying degrees of intestinal resection.Materials and Methods. Male juvenile (70 ± 10 g) and adult (350 ± 30 g) rats of the Holtzman and Sprague Dawley stocks were used. Two stocks of animals were used because not enough animals of one stock could be obtained at the time of the experiment.The animals underwent transection, resection of the small intestine, or a simple laparotomy. The procedures were the same as described elsewhere (2). The mid-portion of the combined ileum and jejunum was transected or resected as noted below and in Table 11. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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