| Autor: |
Yamamoto, Keizou, Ueno, Yuji, Otsubo, Kazumasa, Yamane, Hiroyuki, Komatsu, Ken-ichi, Tani, Yoshiki |
| Zdroj: |
Journal of Fermentation and Bioengineering; January 1992, Vol. 73 Issue: 2 p125-129, 5p |
| Abstrakt: |
Use of peptone and yeast extract as the nitrogen source together with glucose as the carbon source was effective in the culture of Corynebacteriumsp. C5 cells that convert trans-1,4-dicyanocyclohexane (t-DCC) to trans-4-cyanocyclohexane-1-carboxylic acid (t-MCC). The ratio of nitrile hydratase to amidase that converted t-DCC to t-MCC was 3 : 1, but the stability of the nitrile hydratase was one-tenth that of the amidase. Potassium phosphate buffer (pH 8.5) stabilized the two enzymes and produced maximum activity. High yields of t-MCC (99.4%) were produced from a high concentration of t-DCC (40%) in a resting cell system. t-DCC crystals disappeared, with the solution becoming clear as the reaction proceeded. The tranexamic acid synthesized from the t-MCC produced by the resting cells was of very high quality; 99.97% trans-isomer. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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