| Autor: |
Mallery, Donna L., Tanganelli, Bianca, Colella, Stefano, Steingrimsdottir, Herdis, van Gool, Alain J., Troelstra, Christine, Stefanini, Miria, Lehmann, Alan R. |
| Zdroj: |
American Journal of Human Genetics; January 1998, Vol. 62 Issue: 1 p77-85, 9p |
| Abstrakt: |
Cockayne syndrome is a multisystem sun-sensitive genetic disorder associated with a specific defect in the ability to perform transcription-coupled repair of active genes after UV irradiation. Two complementation groups (CS-A and CS-B) have been identified, and 80% of patients have been assigned to the CS-B complementation group. We have analyzed the sites of the mutations in the CSBgene in 16 patients, to determine the spectrum of mutations in this gene and to see whether the nature of the mutation correlates with the type and severity of the clinical symptoms. In nine of the patients, the mutations resulted in truncated products in both alleles, whereas, in the other seven, at least one allele contained a single amino acid change. The latter mutations were confined to the C-terminal two-thirds of the protein and were shown to be inactivating by their failure to restore UV-irradiation resistance to hamster UV61 cells, which are known to be defective in the CSBgene. Neither the site nor the nature of the mutation correlated with the severity of the clinical features. Severe truncations were found in different patients with either classical or early-onset forms of the disease. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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