Rapid identification of Streptococcus pneumoniae by PCR amplification of ribosomal DNA spacer region

Autor: Saruta, Katsutoshi, Matsunaga, Teiichi, Hoshina, Sadayori, Kono, Midori, Kitahara, Shintaro, Kanemoto, Sonomi, Sakai, Osamu, Machida, Katsuhiko
Zdroj: FEMS Microbiology Letters; October 1995, Vol. 132 Issue: 1-2 p165-165, 1p
Abstrakt: Streptococcus pneumoniae is one of the important human pathogens in clinical microbiology. A polymerase chain reaction assay was designed to detect and identify S. pneumoniae through amplification of the ribosomal DNA spacer regions between the pneumococcal 16S-23S ribosomal RNA genes. Thirty-two Streptococcus and non-Streptococcus strains were tested to verify the specificity of the assay, and only S. pneumoniae strains gave a positive reaction. This method is a powerful technique for the rapid identification of S. pneumoniae.
Databáze: Supplemental Index