| Abstrakt: |
The water-insolubilization mechanism of exogenous primer dextran with 1,3-α-d-glucan synthase (EC 2.4.1.-) from Streptococcus mutans was studied. The 1,3-α-d-glucan synthase solution, containing sucrose and exogenous primer dextran, was incubated briefly. Water-insoluble glucan was synthesized. At the same time, water-soluble glucan, mainly derived from exogenous primer dextran, decreased. Linkage analysis data of glucan produced revealed that 1,3-α-d-glucoside bonds increased. Exogenous primer dextran was changed by the action of 1,3-α-d-glucan synthase to water-insoluble glucan. The results suggest that in a short-term reaction system of outside primer-insertion type, the 1,6-α-d-glucoside bond forms the main chain of water-insoluble glucan. |
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