| Autor: |
Kaplanis, J. N., Monroe, R. E., Robbins, W. E., Louloudes, Spiro J. |
| Zdroj: |
Annals of the Entomological Society of America; March 1963, Vol. 56 Issue: 2 p198-198, 1p |
| Abstrakt: |
Adult house flies (Musca domestica L.) were maintained on a semidefined diet containing 0.1% H3-ß-sitosterol and their eggs collected over a 22-day period. Although the major portion of the radioactive compounds in both the adult flies and eggs behaved as free sterols, as much as 30% of the H3 compounds in the eggs was esterified. Column chromatographic analyses of the free sterol and ester fractions indicated the presence of two major radioactive peaks. When the largest peak, which accounted for 88% to 95% of the total H3 compounds, was analyzed by gas-liquid chromatography and reverse-isotope dilution, more than 90% of the radioactivity was found to represent ß-sitosterol. No conversion of ß-sitosterol to cholesterol was detected. The minor peak, which contained 5,7-dienes, was tentatively identified as 7-dehydro-ß-sitosterol. These results indicate that the house fly utilizes ß-sitosterol directly and as a precursor to 7-dehydro-ß-sitosterol without detectable conversion to cholesterol. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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