| Autor: |
Penas, Clara, Govek, Eve-Ellen, Fang, Yin, Ramachandran, Vimal, Daniel, Mark, Wang, Weiping, Maloof, Marie E., Rahaim, Ronald J., Bibian, Mathieu, Kawauchi, Daisuke, Finkelstein, David, Han, Jeng-Liang, Long, Jun, Li, Bin, Robbins, David J., Malumbres, Marcos, Roussel, Martine F., Roush, William R., Hatten, Mary E., Ayad, Nagi G. |
| Zdroj: |
Cell Reports; 20240101, Issue: Preprints |
| Abstrakt: |
Although casein kinase 1δ (CK1δ) is at the center of multiple signaling pathways, its role in the expansion of central nervous system progenitor cells is unknown. Using mouse cerebellar granule cell progenitors (GCPs) as a model for brain neurogenesis, we demonstrate that the loss of CK1δ or treatment of GCPs with a highly selective small molecule inhibits GCP expansion. In contrast, CK1δ overexpression increases GCP proliferation. Thus, CK1δ appears to regulate GCP neurogenesis. CK1δ is targeted for proteolysis via the anaphase-promoting complex/cyclosome (APC/CCdh1) ubiquitin ligase, and conditional deletion of the APC/CCdh1activator Cdh1in cerebellar GCPs results in higher levels of CK1δ. APC/CCdh1also downregulates CK1δ during cell cycle exit. Therefore, we conclude that APC/CCdh1controls CK1δ levels to balance proliferation and cell cycle exit in the developing central nervous system. Similar studies in medulloblastoma cells showed that CK1δ holds promise as a new therapeutic target. |
| Databáze: |
Supplemental Index |
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