Site Directed Antisera to the D-2 Polypeptide Subunit of Photosystem II

Autor: Geiger, R., Berzborn, R. J., Depka, B., Oettmeier, W., Trebst, A.
Zdroj: Zeitschrift für Naturforschung C; April 1987, Vol. 42 Issue: 4 p491-498, 8p
Abstrakt: Three synthetic oligopeptides were used for preparation of antibodies against the D-2 polypeptide of thylakoid membranes. Their sequence was chosen from a model of the folding of the amino acid sequence of the D-2 polypeptide subunit through the membrane that predicted these sequences to be exposed at the membrane surface. For the Merrifield solid-phase method on a fully automated synthesizer the Na-amino group was protected by a fluorenyl-9-methylcarbonyl group. The oligopeptides were coupled to serum albumin by EDAC for immunizations in rabbits.
Databáze: Supplemental Index