| Autor: |
Picco, Natalia Y., Alustiza, Fabrisio E., Bellingeri, Romina V., Grosso, María C., Motta, Carlos E., Larriestra, Alejandro J., Vissio, Claudina, Tiranti, Karina I., Terzolo, Horacio R., Moreira, Ana R., Vivas, Adriana B. |
| Zdroj: |
Revista Argentina de Microbiologia; April-June 2015, Vol. 47 Issue: 2 p95-102, 8p |
| Abstrakt: |
The aim of this study was to perform a current molecular characterization of bovine pathogenic Escherichia colistrains isolated from random samplings in Argentinean dairy farms. Rectal swabs were obtained from 395 (63.7%) healthy and 225 (36.3%) diarrheic calves, belonging to 45 dairy farms in Cordoba Province, Argentina. E. coliisolates were examined for virulence genes (f5, f41, f17, sta, stb, lt, eae, vt) using PCR and the prevalence of E. colivirulence profiles was spatially described in terms of spatial distribution. A total of 30.1% isolates were found to be positive for at least one of the virulence genes. Depending on the different gene combinations present, 11 virulence profiles were found. Most of the isolates analyzed had a single gene, and no combination of fimbrial and enterotoxin gene was predominant. There was no association between the frequency and distribution of E. colivirulence genes and calf health status. Most of the virulence profiles were compatible with ETEC strains and showed a homogeneous distribution over the sampled area. A clustering pattern for E. colivirulence profiles could not be recognized. This work provides updated information on the molecular characterization of pathogenic E. colistrains from dairy herds in Cordoba, Argentina. These findings would be important to formulate prevention programs and effective therapies for diarrhea in calves caused by E. coli. |
| Databáze: |
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